Ion Exchange (IEX) Columns
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Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. These columns are used for the separation of proteins, peptides, and nucleic acids. BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. These columns are available in QA and SP chemistries and are based on porous (QA and SP columns) and non-porous (QF and SF columns) hydrophilic polymer beads with low nonspecific adsorption.
They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns.
Monoclonal antibody (MAb) analysis on non-porous type cation exchange columns
Excellent batch-to-batch reproducibility
30 mm-length column for high-throughput analysis with low operating pressure
High binding capacity and high recovery of biomolecules
High loadability
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(formerly)
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BioPro IEX QA
(YMC-BioPro QA)
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BioPro IEX SP
(YMC-BioPro SP)
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BioPro IEX QF
(YMC-BioPro QA-F)
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BioPro IEX SF
(YMC-BioPro SP-F)
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| Matrix | Hydrophilic polymer (polymethacrylate) | |||||
| Particle Size | 5 µm | 3, 5 µm | ||||
| Pore Size | 100 nm / 1000 Å | Non-porous | ||||
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- | -CH2N+(CH3)3 | -(CH2)3SO3- | ||
| Counter ion | Cl- | Na+ | Cl- | Na+ | ||
| Ion-exchange capacity | 0.075-0.100 meq/ml-resin | 0.070-0.095 meq/ml-resin | 0.075-0.110 meq/ml-resin | 0.230-0.290 meq/ml-resin | ||
| Dynamic binding capacity | 110-150 mg BSA/ml-resin | 70-100 mg human IgG/ml-resin | 12-20 mg BSA/ml-resin | 10-18 mg human IgG/ml-resin | ||
| pH range | 2.0 - 12.0 | |||||
|
(formerly)
|
BioPro IEX QA
(YMC-BioPro QA)
|
BioPro IEX SP
(YMC-BioPro SP)
|
||
| Matrix | Hydrophilic polymer (polymethacrylate) | |||
| Particle Size | 5 µm | |||
| Pore Size | 100 nm / 1000 Å | |||
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- | ||
| Counter ion | Cl- | Na+ | ||
| Ion-exchange capacity | 0.075-0.100 meq/ml-resin | 0.070-0.095 meq/ml-resin | ||
| Dynamic binding capacity | 110-150 mg BSA/ml-resin | 70-100 mg human IgG/ml-resin | ||
| pH range | 2.0 - 12.0 | |||
|
(formerly)
|
BioPro IEX QF
(YMC-BioPro QA-F)
|
BioPro IEX SF
(YMC-BioPro SP-F)
|
| Matrix | Hydrophilic polymer (polymethacrylate) | |
| Particle Size | 3, 5 µm | |
| Pore Size | Non-porous | |
| Charged group | -CH2N+(CH3)3 | -(CH2)3SO3- |
| Counter ion | Cl- | Na+ |
| Ion-exchange capacity | 0.075-0.110 meq/ml-resin | 0.230-0.290 meq/ml-resin |
| Dynamic binding capacity | 12-20 mg BSA/ml-resin | 10-18 mg human IgG/ml-resin |
| pH range | 2.0 - 12.0 | |
| Title | Document Type | Language | Phase Type | Download |
| HPLC Analysis of Oligonucleotides with BioPro IEX QF | Application Note | E | Download | |
| Optimisation of oligonucleotide separations using YMC´s non porous anion exchanger BioPro IEX QF | Application Note | E | Download | |
| YMC Biochromatography Columns | Brochure | E | Download | |
| YMC Biochromatography Columns (HQ) | Brochure | E | Download | |
| BioPro IEX Columns Care and Use | Care and Use Instructions | E | Download | |
| Analytical stationary phases for BioLC from YMC | Overview | E | Download | |
| The Principles of BioLC & YMC BioLC Phase Overview | Poster | E | Download | |
| Characterisation of charge heterogeneity of therapeutic monoclonal antibodies via IEX-MS using BioPro IEX SF from YMC | Product Information | E | Download |