Size Exclusion (SEC) Columns
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YMC-Pack Diol is available in four tightly controlled porosities and is, therefore, suitable for separation and molecular weight determination of a wide range of peptides, proteins, oligonucleotides, carbohydrates and other biopolymers with molecular weights of approx 200 to several hundred thousand.
YMC-SEC MAB is especially designed for the analysis of antibodies, their fragments and aggregates ro provide high resolution for the high and low molecular weight range.
The silica is derivatised with 1,2-dihydroxypropane and, with the variety of pore size available, allows analyses over a broad range of peptide to large proteins or antibodies.
High flexibility
Full scalability
Excellent reproducibility
Cost-efficiency
| YMC-Pack Diol |
YMC-Pack
Diol-60
|
YMC-Pack
Diol-120
|
YMC-Pack
Diol-200
|
YMC-Pack
Diol-300
|
YMC-SEC MAB
|
| Suitable for | for carbohydrates & peptides | for intermediate proteins | for large proteins | for very large proteins | for antibodies, fragments & aggregates |
| Recommended MW Range | <10 kDa | 5 – 100 kDa | 5 – 300 kDa | 20 – 1,000 kDa | 10 - 700 kDa |
| Pore Size [nm / Å] | 6 / 60 | 12 / 120 | 20 / 200 | 30 / 300 | 25 / 250 |
| Particle Size [µm] | 3, 5 | 3, 5 | 2, 3, 5 | 2, 3, 5 | 3 |
| Recommended pH Range | 5.0 - 7.5 | ||||
| Maximum Temperature | 40°C | ||||
YMC-Pack Diol stationary phases are surface modified for a versatile alternative to underivatised silica. Advantages include a shorter equilibration time as well as good selectivity. Due to this short equilibration time, the Diol phase is a good choice for SEC in comparison with underivatised silica.
| Title | Document Type | Language | Phase Type | Download |
| SEC Analysis of MAb Fragments with Increased Throughput and High Resolution | Application Note | E | Download | |
| YMC Biochromatography Columns | Brochure | E | Download | |
| YMC Biochromatography Columns (HQ) | Brochure | E | Download | |
| YMC-Pack Diol SEC (HPLC/UHPLC) Care and Use | Care and Use Instructions | E | Download | |
| YMC-SEC MAB Care and Use | Care and Use Instructions | E | Download | |
| Größenausschluss-Säulen für Antikörper: YMC-SEC MAB | Flyer | D | Download | |
| Size Exclusion Columns for Antibodies: YMC-SEC MAB | Flyer | E | Download | |
| UHPLC Size Exclusion Columns YMC-Pack Diol | Flyer | E | Download | |
| Analytical stationary phases for BioLC from YMC | Overview | E | Download | |
| The Principles of BioLC & YMC BioLC Phase Overview | Poster | E | Download |