YMC-Pack ProFamily

YMC ProFamily is based on a silica with an extremely low metal content which suppresses polar interactions, as metal impurities cause silanol groups to become more acidic. This provides YMC ProFamily excellent resolution for a wide range of compounds, especially basic pharmaceuticals.

Pro series feature advanced endcapping technology and quality assurance which is ready for validation, and they consist 5 types of columns including three kind of ODS each with unique separation characteristics, C8 and C4.

Ultra high purity silica

Extremely low metal content

Advanced endcapping technology

Excellent resolution

Reproducibility

  YMC-Pack Pro C18 YMC Hydrosphere C18 YMC-Pack Pro C8 YMC-Pack Pro C4 YMC-Pack Pro C18 RS
Stationary Phase C18 (USP L1) C18 (USP L1) C8 (USP L7) C4 (USP L26) C18 (USL L1)
Particle Size [µm] 2, 3, 5 2, 3, 5 3, 5 3, 5 3, 5
Pore Size
[nm / Å]
12 / 120 12 / 120 12 / 120 12 / 120 8 / 80
Bonding Type monomeric monomeric monomeric monomeric polymeric
Endcapping Yes
Carbon Load 16% 12% 10% 7% 22%
Surface area [m²/g] 330 330 330 330 510
pH Range 2.0 - 8.0 2.0 - 8.0 2.0 - 7.5 2.0 - 7.5 1.0 - 10.0
Max. Temperature recommended 20-40 °C (upper limit 50°C)
Particle Size 2 µm, 3 µm, 5 µm
Pore Size 12 nm / 120 Å
Carbon Load 16%
Surface area 330 m2/g
pH Range 2-8
Max. temperature recommended 20-40° C (upper limit 50°C)
Specified metal content randomly selected lot:
Al < 10 ppm 0.3
Fe < 10 ppm 2.8
Na < 10 ppm 0.3
Ti < 10 ppm 0.1

YMC-Pack Pro C18 is based on an ultra pure silica support, which is used for the whole ProFamily. Due to a proprietary endcapping process especially designed for this type of silica, YMC-Pack Pro C18 is perfectly suitable for the separation of acidic and basic molecules. The inertness of the silica makes it an excellent choice for the analysis of drugs or metabolites, compounds that are susceptible to polar interactions with residual silanol groups and metal impurities.

YMC-Pack Pro C18 is also available in 2 µm particle size: YMC UltraHT Pro 18. This allows the medium balance of hydrogen-bonding capacity and hydrophobicity of this phase, to be used for ultra-fast LC.

Particle Size 2 µm, 3 µm, 5 µm
Pore Size 12 nm / 120 Å
Carbon Load 12%
Surface area 330 m2/g
pH Range 2-8
Max. temperature recommended 20-40° C (upper limit 50°C)
AQ Stability 100% H2O eluents
Specified metal content randomly selected lot:
Al < 10 ppm 0.7
Fe < 10 ppm 1.2
Na < 10 ppm 0.7
Ti < 10 ppm 0.1

The separation of polar compounds in many cases requires highly aqueous mobile phase conditions to achieve sufficient retention on the stationary phase. Conventional reversed phase selectivities do not give reproducible results under these conditions mainly due to the collapse of the C18 chains. Hydrosphere C18 has been developed, on the ultra pure silica support of the ProFamily, to be stable in 100% aqueous eluents.

For ultra-fast LC applications YMC-Ultra HT Hydrosphere C18 with 2 µm particle size provides the optimal selectivity for the separation of highly polar compounds.

Particle Size 3 µm, 5 µm
Pore Size 12 nm / 120 Å
Carbon Load 10%
Surface area 330 m2/g
pH Range 2-7.5
Max. temperature recommended 20-40° C (upper limit 50°C)
Specified metal content randomly selected lot:
Al < 10 ppm 0.2
Fe < 10 ppm 2.5
Na < 10 ppm 1.4
Ti < 10 ppm 0.1

YMC-Pack Pro C8 is one of YMC’s most commonly used bonded phases and an excellent alternative to C18 selectivities. Due to its moderate hydrophobicity, retention times tend to be shorter than those for ODS phases. YMC-Pack Pro C8 is suitable for a wide range of sample types including pharmaceuticals and biologicals with a relatively high hydrophobicity.

Particle Size 3 µm, 5 µm
Pore Size 12 nm / 120 Å
Carbon Load 7%
Surface area 330 m2/g
pH Range 2-7.5
Max. temperature recommended 20-40° C (upper limit 50°C)
Specified metal content randomly selected lot:
Al < 10 ppm 0.6
Fe < 10 ppm 2.9
Na < 10 ppm 1.0
Ti < 10 ppm 0.1

With Pro C4, YMC offers a stationary phase based on the well-known ultra pure silica of the ProFamily. Compared to a C18 phase with the same eluent, C4 phases are less hydrophobic and gives shorter retention times for non-polar compounds while the retention time of polar analytes are virtually unaffected. This makes the Pro C4 an interesting alternative especially when short analysis times are required.

Particle Size 3 µm, 5 µm
Pore Size 8 nm / 80 Å
Carbon Load 22%
Surface area 510 m2/g
pH Range 1-10
Max. temperature recommended 20-40° C (upper limit 50°C)
Specified metal content randomly selected lot:
Al < 10 ppm 0.3
Fe < 10 ppm 0.1
Na < 10 ppm 1.3
Ti < 10 ppm 0.1

The relatively high carbon load of 22% for YMC-Pack Pro C18 RS and its smaller pore diameter amplifies the selectivity’s ability to discriminate between closely related compounds such as positional or steric isomers. YMC-Pack Pro C18 RS recognises even slight steric differences, even if the analytes differ only in their three-dimensional structure and not in their hydrophobicity or polarity. A more conventional carbon load (~15%) C18 chemistry is not able to use this property for separation.

Title Document Type Language Phase Type Download
High resolution analysis of oligonucleotides on RP chromatography Application Note E Download
YMC Application Collection Pesticides: Achiral and Chiral Applications Application Note E Download
YMC microLC Brochure E Download
YMC-Pack ProFamily incl. Hydrosphere C18 Care and Use Care and Use Instructions E Download
YMC-UltraHT Pro C18, Hydrosphere C18 Care and Use Care and Use Instructions E Download
MSDS Silica based NP/RP Bulk MSDS E Download
Analytical stationary phases for HPLC and UHPLC from YMC Overview E Download
Easy ProFamily Column Selection for Method Development (Radar Chart) Poster E Download
YMC Classic Columns eCatalogue E Download
YMC ProFamily - YMC Classic Columns eCatalogue Chapter E Download