Contichrom CUBE
Contichrom CUBE is a flagship FPLC system which is built on YMC Intelligence. It offers unique capabilities for mastering complex separation challenges by using our proprietary processes. The user-friendly software automates the use of these processes.
The Contichrom CUBE Combined is available in two versions: Contichrom CUBE Combined 30: flow rate range 0.1 – 36 mL/min Contichrom CUBE Combined 100: flow rate range 0.1 – 100 mL/m
Standard batch processes
Automated two-step batch processes with in-line dilution
Automated cyclic processes for optimized capture (CaptureSMB) for antibodies and tagged proteins
AutomAb dynamic process control for keeping the CaptureSMB process at an optimum
Automated cyclic polishing process (MCSGP) for difficult separation challenges
MControl dynamic process control for keeping the MCSGP process at an optimum
Automated cyclic enrichment process (N-Rich) allowing to isolate product-related impurities
Fraction collector
Optional: cooling chamber
Feed and eluate can be kept cooling
| Process capabilities and process control | Batch (isocratic, gradient), two-step integrated batch (isocratic, gradient), CaptureSMB (optional), MCSGP, N-Rich, AutomAb (optional), MControl • Full capabilities: batch (isocratic, gradient), two-step integrated batch (isocratic, gradient), CaptureSMB (optional), MCSGP, N-Rich, AutomAB (optional), MControl, • Recommended for synthetic peptide purification: batch (isocratic, gradient), MCSGP, N-Rich • Recommended for Omega-3 EPA purification: batch (isocratic, gradient), MCSGP, N-Rich • Process capabilities can be customized according to purification challenges |
| Operating software | User-friendly operating software ChromIQ with step-by-step wizards to help you to design both standard batch and two-step integrated batch chromatography processes and to convert them into more efficient continuous chromatography Contichrom processes, such as MCSGP and N-Rich. ChromIQ also includes the MControl process control function for MCSGP keeping the process at the optimum. |
| Software compliance | ChromIQ software with all essential elements of 21CFR Part 11 compliance: • Pre-defined user groups, administrators, R&D and production users • Rights management for individual user groups • User accounts are password protected • Logging with time stamp and user name (non-deletable) • Electronic signature with checksum of log and measurement files |
| Pressure rating | 100 bar (10 MPa / 1450 psi) |
| Flow rate range | 0.1–36 mL/min (Contichrom HPLC 30) 0.1–100 mL/min (Contichrom HPLC 100) |
| Buffer selection | 18 inlets (2x 8-fold buffer selection valve, 2 additional inlets) 4 outlets |
| UV detector | 2 long lifetime LED UV detectors, each with 254 & 280 nm recorded simultaneously (fixed wavelengths) Optional upgradable with external multi-wavelength UV-vis detectors (190–500 nm or 190–700 nm) |
| Conductivity detector | 2 conductivity sensors (1–150 mS/cm) |
| pH detector range | 1–14 |
| Pump type | 4 high precision double-piston-pumps with active seal wash |
| Valves | 6 reliable multi-position valves Optional 6, 12 or 16 port sample collection valve for large volume sample collection |
| Computer | Stand-alone laptop computer (Windows, 64 bit, full HD resolution, 1920 x 1080 or higher) with ChromIQ software |
| Other | Cold room compatible Large buffer tray Portable & compact Runs resins and membrane stationary phases In-line dilution capability Back-to-base servicing |
| Dimensions (height x width x depth) | Consists of two modules: 370 mm x 509 mm x 450 mm (14.6" x 20.0" x 17.7") 214 mm x 509 mm x 450 mm (8.43" x 20.0" x 17.7") |
| Weight | Consists of two modules: 30 kg (67 lb) 17 kg (38 lb) Total: 47 kg (105 lb) |
| Materials | All biocompatible High pressure side capillaries: PEEK Low pressure side tubing: PTFE Fittings: PEEK Solvent compatibility with all standard HPLC solvents |