Hydrophobic Interaction (HIC) Columns

Hydrophobic Interaction Chromatography Column, is based on a non-porous hydrophobic polymer and is effective for separation of proteins and antibodies such as Antibody-Drug Conjugates (ADCs). It is designed and optimised for analysis and lab-scale purification of antibodies, antibody-drug conjugates and other proteins.

Optimised surface modification and column packing method realise high separation performance. High-throughput analysis with a faster flow rate can be achieved by the rigid particle and moderate backpressure.

Effective for Drug-to-Antibody Ratio (DAR) analysis of ADCs

High resolution comparable to sub-3 µm with low operating pressure

Fast analysis at high flow rates

Excellent peak shape under high loading

 
BioPro HIC BF
Base particle Hydrophilic polymer (polymethacrylate)
Particle size 4 µm
Pore size Non-porous
Functional group Butyl
pH range 2.0 - 12.0
Temperature range 10 - 60°C
Title Document Type Language Phase Type Download
HIC Analysis of Different Monoclonal Antibodies using Isopropanol as Modifier Application Note E Download
HPLC Analysis of Drug-to-Antibody with BioPro HIC BF by YMC Application Note E Download
YMC Biochromatography Columns Brochure E Download
YMC Biochromatography Columns (HQ) Brochure E Download
BioPro HIC BF, HT Care and Use Care and Use Instructions E
BioPro HIC BF by YMC Flyer E Download
Analytical stationary phases for BioLC from YMC Overview E Download
The Principles of BioLC & YMC BioLC Phase Overview Poster E Download