N Rich

EFFICIENT ISOLATION OF MINOR COMPOUNDS BY N-RICH


The isolation of minor compounds of complex mixtures is a very difficult purification task, typically done by repetitive (several hundred) runs using analytical HPLC in order to isolate mg quantities of a minor compound for further testing. The N-Rich process is an automated multistep process that is run by the Contichrom CUBE Combine and HPLC systems providing mg quantities of a desired compound at high purity. The two-step enrichment and separation step yields sufficient material of high purity for structure elucidation or subsequent structural, biochemical, cell- and even animal-based assays. It is an invaluable tool for any research and development laboratory.

N-Rich is applied for:

  • Isolation of product-related impurities for active pharmaceutical ingredients
  • Biosimilar CMC characterization
  • Characterization of antibody-drug conjugate isoforms
  • Identification of biomarkers and compounds in proteomics and metabolomics research
  • Purification of ultra-pure compounds as reference materials
  • Isolation of impurities after stability and formulation studies

    • The N-Rich process employs a cyclic step where the desired molecule is selectively enrichment and interfering substances are depleted (see animation below). After enrichment of several hundred to thousand-fold, the enriched fraction is separated automatically, using a shallow gradient.

The Contichrom CUBE Combined and the Contichrom HPLC are capable of executing N-Rich processes.